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KMID : 1149220090170020163
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Korean Journal of Oriental Medical Prescription
2009 Volume.17 No. 2 p.163 ~ p.174
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Characterization of polysaccharide A-1 from Opuntia ficus-indica and it’s protection effect on alcoholic induced hepatic oxidative stress
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Ryu Il-Hwan
Gwon Ji-Woong
Lee Eoh-Jin
Yun Young-Gab
Gwon Tae-Oh
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Abstract
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Reactive oxygen species(ROS) can induce hepatotoxicity and trigger apoptosis in the liver. In this study, we investigated the sulfated polysaccharide A-1 from Opuntia ficus-indica against alcoholic oxidative stress in human liver Hep G2 cell. An antioxidant substance A-1 obtained from the enzymatic extract of Opuntia ficus-indica fruit was purified by DEAE-cellulose ion exchange and sephadex G-100 gel permeation chromatography. The purification yield and molecular weight were 14.3% and 1.8 KDa, respectively. The A-1 predominately contained arabinose, galactose, rhamnose and also sulfate group. The structure of A-1 was investigated by periodate oxidation, FT-IR spectroscopy, 1H-NMR spectroscopy. The A-1 mainly composed of alternating unit of →4)-α-L- Rapp-2-SO3--α-L-Galp-(1→ and branched linkage of β-D-Arbp- (5→. The antioxidative activity was measured using the SOD, CAT activity and GSH assay, respectively. The expression of Nrf2 protein was analyzed by western blotting. The viable cell count analyzed by autofluorescence. Oxidative stress induced by ethanol(1 M) were dramatically reduced by A-1 treatment. A-1 also prevented cell death induced by oxidative stress. It also increased expression Nrf2 protein level. We concluded that sulfated polysaccharide A-1 from Opuntia ficus-indica effectively protect Hep G2 liver cell from alcoholic oxidative stress.
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KEYWORD
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Opuntia ficus-indica, Alcoholic oxidative stress, Hepatotoxicity, Hep G2
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